Differential expression of CCR2 and CX 3 CR1 on CD16 + monocyte subsets is associated with asthma severity

• Published in: Allergy, asthma, and clinical immunology Vol. 15; p. 64
• Main Authors: Al-Rashoudi, Reem, Moir, Gillian, Al-Hajjaj, Mohamed S, Al-Alwan, Monther M, Wilson, Heather M, Crane, Isabel J
• Format: Journal Article
• Language: English
• Published: England 2019
• Subjects: Saudi Arabia; Flow cytometry; Biomarker; Chemokine receptor; CD14
• ISSN: 1710-1484

Monocytes play an important role in immune and inflammatory diseases and monocyte subsets are predictors of disease in certain conditions. Expression of the chemokine receptors, CCR2 and CX CR1 on monocyte subsets relates to their function and can be used in their characterization. Our objective was to determine whether CD14, CD16, CCR2 and CX CR1 on monocyte subsets are potential indicators of asthma severity. Blood samples were collected from Saudi Arabian patients with asthma and normal healthy individuals. Six-color flow-cytometry phenotypic analysis was used to identify human blood monocyte subsets, based on their expression of CD14 and CD16 following CD45 gating. Expression of CCR2 and CX CR1 was analysed on classical (CD14 CD16 ), intermediate (CD14 CD16 ) and non-classical (CD14 CD16 ) subsets and correlated with disease severity. We demonstrated a significant increase in percentage of total CD45-positive monocytes in the blood of patients with severe asthma, but the proportion of the individual monocyte subsets was not significantly changed when patients with mild, moderate and severe asthma were compared with healthy individuals. CD16 expression (mean fluorescence intensity, MFI) was decreased on intermediate and non-classical subsets in patients with severe asthma compared to healthy controls. CX CR1 expression was also lower, with a lower percentage of cells expressing CX CR1 in the non-classical CD14 CD16 subset in all patients with asthma and this was inversely related to the percentage of cells expressing CCR2. CCR2 expression on monocytes indicated a tendency toward more phagocytic monocytes in patients with asthma. The differential expression of CD16, CX CR1 and CCR2 on monocyte subsets in peripheral blood indicates modulation of the inflammatory response and suggests a role for monocytes in asthma pathogenesis.