Antileishmanial activity and immunomodulatory effect of seco subamolide, a butanolide isolated from Nectandra oppositifolia (Lauraceae)

• Published in: The journal of venomous animals and toxins including tropical diseases Vol. 25; p. e20190008
• Main Authors: da Costa-Silva, Thais A, Conserva, Geanne A Alves, Galisteo, Jr, Andrés J, Tempone, Andre G, Lago, João Henrique G
• Format: Journal Article
• Language: English
• Published: Brazil 2019
• Subjects: Nectandra oppositifolia; Antileishmanial; Leishmania (L.) infantum chagasi; Secosubamolide; Immunomodulatory
• ISSN: 1678-9199; 1678-9199

Visceral leishmaniasis is a complex neglected tropical disease caused by complex. Its current treatment reveals strong limitations, especially high toxicity. In this context, natural products are important sources of new drug alternatives for VL therapy. Therefore, the antileishmanial and immunomodulatory activity of compounds isolated from (Lauraceae) was investigated herein The -hexane extract from twigs of were subjected to HPLC/HRESIMS and bioactivity-guided fractionation to afford compounds and which were evaluated against ( ) and NCTC cells. The -hexane extract displayed activity against ( ) and afforded linderanolide E ( ) and subamolide A ( ), which were effective against ( ) promastigotes, with IC values of 57.9 and 24.9 µM, respectively. Compound was effective against amastigotes (IC = 10.5 µM) and displayed moderate mammalian cytotoxicity (CC = 42 µM). The immunomodulatory studies of compound suggested an anti-inflammatory activity, with suppression of IL-6, IL-10, TNF with lack of nitric oxide. This study showed the antileishmanial activity of compounds and isolated from . Furthermore, compound demonstrated an antileishmanial activity towards amastigotes associated to an immunomodulatory effect.