The Oxidized proteins and activity of the Cl-/HCО3- exchanger in erythrocytes of patients with acute alcohol intoxication

The purpose of this research was to study the morphological properties and the products of oxidative protein modification in erythrocytes of patients with acute alcohol intoxication. Two groups of subjects were analyzed. The first one included 39 patients with acute alcohol intoxication. The second...

Full description

Saved in:
Bibliographic Details
Published inActa biochimica Polonica Vol. 66; no. 3; p. 351
Main Authors Demidchik, Lyudmila, Kolesnikova, Yevgeniya, Muravlyova, Larissa, Molotov-Luchanskiy, Vilen, Kluyev, Dmitriy, Bakirova, Ryszhan, Omarova, Indira, Ponamareva, Olga, Tankibaeva, Neila, Nukhuly, Altynbek
Format Journal Article
LanguageEnglish
Published Poland 19.08.2019
Subjects
Adult
Alcoholic Intoxication - metabolism
Carrier Proteins - metabolism
Cell Size - drug effects
Chloride-Bicarbonate Antiporters - metabolism
Erythrocytes - metabolism
Ethanol - pharmacology
Female
Hemoglobins - metabolism
Hemolysis - drug effects
Humans
Ion Transport
Kazakhstan
Male
Membrane Proteins - metabolism
Middle Aged
Oxidation-Reduction
Reactive Oxygen Species - metabolism
Young Adult
Kazakhstan
Online AccessGet more information

Cover

More Information
Summary:The purpose of this research was to study the morphological properties and the products of oxidative protein modification in erythrocytes of patients with acute alcohol intoxication. Two groups of subjects were analyzed. The first one included 39 patients with acute alcohol intoxication. The second group consisted of 14 healthy subjects. In erythrocytes the activity of Cl-/HCО3- exchanger, the reactive protein carbonyl derivatives and membrane-bound hemoglobin concentration were measured. Our results demonstrated strong alteration of the Cl-/HCО3- exchanger activity in erythrocytes of patients with acute alcohol intoxication. A delay in the beginning of hemolysis during incubation of erythrocytes in the ammonium medium was observed. The concentration of protein carbonyls in erythrocytes of patients significantly increased in comparison to the control ones. A decrease in the membrane-bounded hemoglobin was observed as well. These findings indicate that ethanol toxicity is manifested by alteration of oxidized protein concentration and Cl-/HCО3- exchanger activity in erythrocytes. It is hypothesized that oxidized proteins are implicated in modulation of the erythrocyte cell volume regulation.
ISSN:1734-154X