Sequencing of Porphyromonas gingivalis from saliva in patients with periodontitis and type 2 diabetes mellitus
Porphyromonas gingivalis is a major periodontal pathogen. Saliva is the most easy, non-invasive microbiological sample for detection of periodontal pathogens. A prospective study on 37 diabetic patients was grouped into well-controlled diabetes with/without periodontitis and uncontrolled diabetic wi...
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Published in | Indian journal of medical microbiology Vol. 37; no. 1; p. 54 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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India
01.01.2019
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Abstract | Porphyromonas gingivalis is a major periodontal pathogen. Saliva is the most easy, non-invasive microbiological sample for detection of periodontal pathogens.
A prospective study on 37 diabetic patients was grouped into well-controlled diabetes with/without periodontitis and uncontrolled diabetic with periodontitis. PCR and sequencing of P. gingivalis was performed in saliva samples.
DNA was extracted from saliva using Triton X-100 and 16s rRNA gene (404 bp) was amplified by polymerase chain reaction. DNA sequencing was performed for two samples.
P. gingivalis was detected in 27.03% (n = 10), of which 30% (n = 9) were diabetic with periodontal disease and 14.3% (n = 1) were diabetic without periodontal disease. The percentage of poor oral hygiene was 50% and 20% in uncontrolled and controlled glycaemic patients, respectively. DNA sequencing of two samples showed 100% identity with the sequences in the GenBank database (Gen Bank accession no: KX640913-KX640914).
Type 2 diabetes mellitus and periodontitis are interlinked. Early detection of P. gingivalis and appropriate treatment with doxycycline will also assist in controlling the glycaemic status. |
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AbstractList | Porphyromonas gingivalis is a major periodontal pathogen. Saliva is the most easy, non-invasive microbiological sample for detection of periodontal pathogens.
A prospective study on 37 diabetic patients was grouped into well-controlled diabetes with/without periodontitis and uncontrolled diabetic with periodontitis. PCR and sequencing of P. gingivalis was performed in saliva samples.
DNA was extracted from saliva using Triton X-100 and 16s rRNA gene (404 bp) was amplified by polymerase chain reaction. DNA sequencing was performed for two samples.
P. gingivalis was detected in 27.03% (n = 10), of which 30% (n = 9) were diabetic with periodontal disease and 14.3% (n = 1) were diabetic without periodontal disease. The percentage of poor oral hygiene was 50% and 20% in uncontrolled and controlled glycaemic patients, respectively. DNA sequencing of two samples showed 100% identity with the sequences in the GenBank database (Gen Bank accession no: KX640913-KX640914).
Type 2 diabetes mellitus and periodontitis are interlinked. Early detection of P. gingivalis and appropriate treatment with doxycycline will also assist in controlling the glycaemic status. |
Author | Mani, Monika Radhakrishnan, Preethi Anbalagan, Rubini Srikanth, Padma Seshadri, Krishna G Barani, Ramya |
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SubjectTerms | Adult Aged Bacteroidaceae Infections - drug therapy Bacteroidaceae Infections - transmission Diabetes Complications - microbiology Diabetes Mellitus, Type 2 - epidemiology Diabetes Mellitus, Type 2 - pathology Doxycycline - therapeutic use Female Glycated Hemoglobin A - analysis Glycemic Index - drug effects Humans India - epidemiology Male Middle Aged Oral Hygiene - statistics & numerical data Periodontitis - drug therapy Periodontitis - epidemiology Periodontitis - microbiology Polymerase Chain Reaction Porphyromonas gingivalis - drug effects Porphyromonas gingivalis - genetics Prospective Studies RNA, Ribosomal, 16S - genetics Saliva - microbiology |
Title | Sequencing of Porphyromonas gingivalis from saliva in patients with periodontitis and type 2 diabetes mellitus |
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