Sequencing of Porphyromonas gingivalis from saliva in patients with periodontitis and type 2 diabetes mellitus

Porphyromonas gingivalis is a major periodontal pathogen. Saliva is the most easy, non-invasive microbiological sample for detection of periodontal pathogens. A prospective study on 37 diabetic patients was grouped into well-controlled diabetes with/without periodontitis and uncontrolled diabetic wi...

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Published inIndian journal of medical microbiology Vol. 37; no. 1; p. 54
Main Authors Radhakrishnan, Preethi, Anbalagan, Rubini, Barani, Ramya, Mani, Monika, Seshadri, Krishna G, Srikanth, Padma
Format Journal Article
LanguageEnglish
Published India 01.01.2019
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Abstract Porphyromonas gingivalis is a major periodontal pathogen. Saliva is the most easy, non-invasive microbiological sample for detection of periodontal pathogens. A prospective study on 37 diabetic patients was grouped into well-controlled diabetes with/without periodontitis and uncontrolled diabetic with periodontitis. PCR and sequencing of P. gingivalis was performed in saliva samples. DNA was extracted from saliva using Triton X-100 and 16s rRNA gene (404 bp) was amplified by polymerase chain reaction. DNA sequencing was performed for two samples. P. gingivalis was detected in 27.03% (n = 10), of which 30% (n = 9) were diabetic with periodontal disease and 14.3% (n = 1) were diabetic without periodontal disease. The percentage of poor oral hygiene was 50% and 20% in uncontrolled and controlled glycaemic patients, respectively. DNA sequencing of two samples showed 100% identity with the sequences in the GenBank database (Gen Bank accession no: KX640913-KX640914). Type 2 diabetes mellitus and periodontitis are interlinked. Early detection of P. gingivalis and appropriate treatment with doxycycline will also assist in controlling the glycaemic status.
AbstractList Porphyromonas gingivalis is a major periodontal pathogen. Saliva is the most easy, non-invasive microbiological sample for detection of periodontal pathogens. A prospective study on 37 diabetic patients was grouped into well-controlled diabetes with/without periodontitis and uncontrolled diabetic with periodontitis. PCR and sequencing of P. gingivalis was performed in saliva samples. DNA was extracted from saliva using Triton X-100 and 16s rRNA gene (404 bp) was amplified by polymerase chain reaction. DNA sequencing was performed for two samples. P. gingivalis was detected in 27.03% (n = 10), of which 30% (n = 9) were diabetic with periodontal disease and 14.3% (n = 1) were diabetic without periodontal disease. The percentage of poor oral hygiene was 50% and 20% in uncontrolled and controlled glycaemic patients, respectively. DNA sequencing of two samples showed 100% identity with the sequences in the GenBank database (Gen Bank accession no: KX640913-KX640914). Type 2 diabetes mellitus and periodontitis are interlinked. Early detection of P. gingivalis and appropriate treatment with doxycycline will also assist in controlling the glycaemic status.
Author Mani, Monika
Radhakrishnan, Preethi
Anbalagan, Rubini
Srikanth, Padma
Seshadri, Krishna G
Barani, Ramya
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  givenname: Rubini
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  surname: Srikanth
  fullname: Srikanth, Padma
  organization: Department of Microbiology, Diabetes and Metabolism, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India
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Keywords Porphyromonas gingivalis
saliva
Glycaemic control
polymerase chain reaction
Language English
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Snippet Porphyromonas gingivalis is a major periodontal pathogen. Saliva is the most easy, non-invasive microbiological sample for detection of periodontal pathogens....
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StartPage 54
SubjectTerms Adult
Aged
Bacteroidaceae Infections - drug therapy
Bacteroidaceae Infections - transmission
Diabetes Complications - microbiology
Diabetes Mellitus, Type 2 - epidemiology
Diabetes Mellitus, Type 2 - pathology
Doxycycline - therapeutic use
Female
Glycated Hemoglobin A - analysis
Glycemic Index - drug effects
Humans
India - epidemiology
Male
Middle Aged
Oral Hygiene - statistics & numerical data
Periodontitis - drug therapy
Periodontitis - epidemiology
Periodontitis - microbiology
Polymerase Chain Reaction
Porphyromonas gingivalis - drug effects
Porphyromonas gingivalis - genetics
Prospective Studies
RNA, Ribosomal, 16S - genetics
Saliva - microbiology
Title Sequencing of Porphyromonas gingivalis from saliva in patients with periodontitis and type 2 diabetes mellitus
URI https://www.ncbi.nlm.nih.gov/pubmed/31424011
Volume 37
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