Sequencing of Porphyromonas gingivalis from saliva in patients with periodontitis and type 2 diabetes mellitus

• Published in: Indian journal of medical microbiology Vol. 37; no. 1; p. 54
• Main Authors: Radhakrishnan, Preethi, Anbalagan, Rubini, Barani, Ramya, Mani, Monika, Seshadri, Krishna G, Srikanth, Padma
• Format: Journal Article
• Language: English
• Published: India 01.01.2019
• Subjects: Adult; Aged; Bacteroidaceae Infections - drug therapy; Bacteroidaceae Infections - transmission; Diabetes Complications - microbiology; Diabetes Mellitus, Type 2 - epidemiology; Diabetes Mellitus, Type 2 - pathology; Doxycycline - therapeutic use; Female; Glycated Hemoglobin A - analysis; Glycemic Index - drug effects; Humans; India - epidemiology; Male; Middle Aged; Oral Hygiene - statistics & numerical data; Periodontitis - drug therapy; Periodontitis - epidemiology; Periodontitis - microbiology; Polymerase Chain Reaction; Porphyromonas gingivalis - drug effects; Porphyromonas gingivalis - genetics; Prospective Studies; RNA, Ribosomal, 16S - genetics; Saliva - microbiology; India; Porphyromonas gingivalis; saliva; Glycaemic control; polymerase chain reaction
• ISSN: 1998-3646

Porphyromonas gingivalis is a major periodontal pathogen. Saliva is the most easy, non-invasive microbiological sample for detection of periodontal pathogens. A prospective study on 37 diabetic patients was grouped into well-controlled diabetes with/without periodontitis and uncontrolled diabetic with periodontitis. PCR and sequencing of P. gingivalis was performed in saliva samples. DNA was extracted from saliva using Triton X-100 and 16s rRNA gene (404 bp) was amplified by polymerase chain reaction. DNA sequencing was performed for two samples. P. gingivalis was detected in 27.03% (n = 10), of which 30% (n = 9) were diabetic with periodontal disease and 14.3% (n = 1) were diabetic without periodontal disease. The percentage of poor oral hygiene was 50% and 20% in uncontrolled and controlled glycaemic patients, respectively. DNA sequencing of two samples showed 100% identity with the sequences in the GenBank database (Gen Bank accession no: KX640913-KX640914). Type 2 diabetes mellitus and periodontitis are interlinked. Early detection of P. gingivalis and appropriate treatment with doxycycline will also assist in controlling the glycaemic status.