Comparative antibacterial activity of fleroxacin: selection frequencies and outer membrane proteins of resistant mutants

The antibacterial activity of fleroxacin was evaluated in 427 gram-positive and gram-negative strains, all isolated recently from clinical specimens and compared to that of ofloxacin, ciprofloxacin and co-trimoxazole. The activity of fleroxacin resembled to that of ofloxacin; its excellent activity...

Full description

Saved in:
Bibliographic Details
Published inImmunitat und Infektion Vol. 16; no. 4; p. 152
Main Authors Cullmann, W, Dechène, M, Stieglitz, M
Format Journal Article
LanguageGerman
Published Germany 01.08.1988
Subjects
Anti-Bacterial Agents - pharmacology
Bacteria - drug effects
Bacteria - genetics
Bacterial Outer Membrane Proteins - analysis
Bacterial Outer Membrane Proteins - genetics
Ciprofloxacin - analogs & derivatives
Ciprofloxacin - pharmacology
Drug Combinations - pharmacology
Drug Resistance, Microbial
Escherichia coli - drug effects
Escherichia coli - genetics
Fleroxacin
Humans
Mutation
Ofloxacin - pharmacology
Serratia marcescens - drug effects
Serratia marcescens - genetics
Sulfamethoxazole - pharmacology
Trimethoprim - pharmacology
Trimethoprim, Sulfamethoxazole Drug Combination
Online AccessGet more information

Cover

More Information
Summary:The antibacterial activity of fleroxacin was evaluated in 427 gram-positive and gram-negative strains, all isolated recently from clinical specimens and compared to that of ofloxacin, ciprofloxacin and co-trimoxazole. The activity of fleroxacin resembled to that of ofloxacin; its excellent activity against Haemophilus influenzae on the one hand and its lack of activity against beta-hemolytic streptococci on the other hand have to be mentioned. Selection frequencies for resistant clones were evaluated for clinical E. coli and Serratia marcescens isolates and the quinolones. With respect to clinical E. coli and Serratia marcescens isolates selection frequencies ranged from 10(-7) to 10(-9) in the presence of 2-fold or 8-fold the MIC. The outer membrane proteins of E. coli and Serratia marcescens wild-type strains were compared with those of their quinolone-resistant mutants. No discrepancies could be observed in E. coli, whereas some of the resistant Serratia marcescens mutants exhibited an increased expression of 31 kdal protein linked with a decrease of a 37 kdal major outer membrane protein. As these alterations could not be observed in each of the resistant mutants, it cannot be decided at present whether such alterations may provide an explanation for the resistance observed.