Mechanistic insights into store-operated Ca 2+ entry during excitation-contraction coupling in skeletal muscle

• Published in: Biochimica et biophysica acta. Molecular cell research
• Main Authors: Koenig, Xaver, Choi, Rocky H, Schicker, Klaus, Singh, Daniel P, Hilber, Karlheinz, Launikonis, Bradley S
• Format: Journal Article
• Language: English
• Published: Netherlands 27.02.2019
• Subjects: Calcium; Electrical field stimulation; Excitation-contraction coupling; Store-operated calcium entry; stim1; orai1; Confocal microscopy; Skinned fibre; Skeletal muscle
• ISSN: 1879-2596

Skeletal muscle fibres support store-operated Ca -entry (SOCE) across the t-tubular membrane upon exhaustive depletion of Ca from the sarcoplasmic reticulum (SR). Recently we demonstrated the presence of a novel mode of SOCE activated under conditions of maintained [Ca ] . This phasic SOCE manifested in a fast and transient manner in synchrony with excitation contraction (EC)-coupling mediated SR Ca -release (Communications Biology 1:31, doi: https://doi.org/10.1038/s42003-018-0033-7). Stromal interaction molecule 1 (STIM1) and calcium release-activated calcium channel 1 (ORAI1), positioned at the SR and t-system membranes, respectively, are the considered molecular correlate of SOCE. The evidence suggests that at the triads, where the terminal cisternae of the SR sandwich a t-tubule, STIM1 and ORAI1 proteins pre-position to allow for enhanced SOCE transduction. Here we show that phasic SOCE is not only shaped by global [Ca ] but provide evidence for a local activation within nanodomains at the terminal cisternae of the SR. This feature may allow SOCE to modulate [Ca ] during EC coupling. We define SOCE to occur on the same timescale as EC coupling and determine the temporal coherence of SOCE activation to SR Ca release. We derive a delay of 0.3 ms reflecting diffusive Ca -equilibration at the luminal ryanodine receptor 1 (RyR1) channel mouth upon SR Ca -release. Numerical simulations of Ca -calsequestrin binding estimates a characteristic diffusion length and confines an upper limit for the spatial distance between STIM1 and RyR1. Experimental evidence for a 4- fold change in t-system Ca -permeability upon prolonged electrical stimulation in conjunction with numerical simulations of Ca -STIM1 binding suggests a Ca dissociation constant of STIM1 below 0.35 mM. Our results show that phasic SOCE is intimately linked with RyR opening and closing, with only μs delays, because [Ca ] in the terminal cisternae is just above the threshold for Ca dissociation from STIM1 under physiological resting conditions. This article is part of a Special Issue entitled: ECS Meeting edited by Claus Heizmann, Joachim Krebs and Jacques Haiech.