Effect of Phosphorus Addition on the Abundance of Autotrophic CO 2 -Fixation Microorganisms in Rhizospheric Soil from a Phosphorus-Limited Paddy Field

• Published in: Huanjing kexue Vol. 39; no. 12; p. 5672
• Main Authors: Bai, Jing, Li, Yi-Fei, Liu, Shou-Long, Zhu, Zhen-Ke, Li, Ke-Lin, Peng, Pei-Qin, Wu, Xiao-Hong, Ge, Ti-da, Wu, Jin-Shui
• Format: Journal Article
• Language: Chinese
• Published: China 08.12.2018
• Subjects: Bacteria; Carbon Dioxide - chemistry; Fertilizers; Oryza; Phosphorus - chemistry; Rhizosphere; Ribulose-Bisphosphate Carboxylase; Soil - chemistry; Soil Microbiology; gene abundance; autotrophic CO2-fixation microorganism; rhizosphere soil; phosphorus-limited paddy soil; phosphorus
• ISSN: 0250-3301

A rice pot experiment was conducted to investigate the effect of phosphorus addition on the abundance of autotrophic CO -fixation microorganisms using phosphorus-limited paddy soil from the Changsha Observation and Research Station for the Agricultural Environment. Rice seedlings were transplanted in the paddy soil with or without phosphorus addition, corresponding to P-treated-pot (P) or control pot (CK), respectively. Rhizosphere soils were collected from the P and CK treatments during the tillering and shooting stages. The physical and chemical soil properties were measured and the abundance of autotrophic CO -fixation microorganisms was quantified with a real-time PCR technique based on four functional genes ( , , , and ) involved in three CO -fixation pathways (CBB cycle, rTCA cycle, and 3-hydroxypropionate/4-hydroxybutyrate cycle). The results show that phosphorus addition improves the concentrations of DOC and Olsen-P and the pH value, whereas negative effects on the MBC and NH -N concentrations are re