Improved cellular uptake of perfluorocarbon nanoparticles for in vivo murine cardiac 19 F MRS/MRI and temporal tracking of progenitor cells

Herein, we maximize the labeling efficiency of cardiac progenitor cells (CPCs) using perfluorocarbon nanoparticles (PFCE-NP) and F MRI detectability, determine the temporal dynamics of single-cell label uptake, quantify the temporal viability/fluorescence persistence of labeled CPCs in vitro, and im...

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Published inNanomedicine Vol. 18; p. 391
Main Authors Constantinides, Christakis, McNeill, Eileen, Carnicer, Ricardo, Al Haj Zen, Ayman, Sainz-Urruela, Raquel, Shaw, Andrew, Patel, Jyoti, Swider, Edyta, Alonaizan, Rita, Potamiti, Louiza, Hadjisavvas, Andreas, Padilla-Parra, Sergi, Kyriacou, Kyriacos, Srinivas, Mangala, Carr, Carolyn A
Format Journal Article
LanguageEnglish
Published United States 01.06.2019
Subjects
Animals
Cell Survival
Cell Tracking
Endocytosis
Female
Fluorescence
Fluorine - chemistry
Fluorocarbons - metabolism
Magnetic Resonance Imaging
Mice, Inbred C57BL
Myocardium - cytology
Nanoparticles - chemistry
Signal-To-Noise Ratio
Stem Cells - metabolism
Time Factors
Fluorine MRI
Tracking
Perfluorocarbon nanoparticles
Cardiac stem cells
Macrophages
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Summary:Herein, we maximize the labeling efficiency of cardiac progenitor cells (CPCs) using perfluorocarbon nanoparticles (PFCE-NP) and F MRI detectability, determine the temporal dynamics of single-cell label uptake, quantify the temporal viability/fluorescence persistence of labeled CPCs in vitro, and implement in vivo, murine cardiac CPC MRI/tracking that could be translatable to humans. FuGENE -mediated CPC PFCE-NP uptake is confirmed with flow cytometry/confocal microscopy. Epifluorescence imaging assessed temporal viability/fluorescence (up to 7 days [D]). Nonlocalized murine F MRS and cardiac MRI studied label localization in terminal/longitudinal tracking studies at 9.4 T (D1-D8). A 4-8 fold F concentration increase is evidenced in CPCs for FuGENE vs. directly labeled cells. Cardiac F signals post-CPC injections diminished in vivo to ~31% of their values on D1 by D7/D8. Histology confirmed CPC retention, dispersion, and macrophage-induced infiltration. Intra-cardiac injections of PFCE-NP-labeled CPCs with FuGENE can be visualized/tracked in vivo for the first time with F MRI.
ISSN:1549-9642