A novel label-free colorimetric detection of l-histidine using Cu 2+ -modulated G-quadruplex-based DNAzymes

We proposed a colorimetric method for l-histidine detection based on Cu -mediated DNAzyme and G-quadruplex-hemin complex catalyzed oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). In this system, after the addition of l-histidine, the formation of G-qu...

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Published inSpectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 203; p. 195
Main Authors Gu, Pan, Zhang, Gehou, Deng, Zhiyi, Tang, Zhenwei, Zhang, Huifang, Khusbu, Farjana Yeasmin, Wu, Kefeng, Chen, Mingjian, Ma, Changbei
Format Journal Article
LanguageEnglish
Published England 05.10.2018
Subjects
DNAzyme
Histidine
G-quadruplex
Colorimetric
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Summary:We proposed a colorimetric method for l-histidine detection based on Cu -mediated DNAzyme and G-quadruplex-hemin complex catalyzed oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). In this system, after the addition of l-histidine, the formation of G-quadruplex-hemin complex will be disturbed, thus the colorimetric signal intensity conversely corresponds to the concentration of histidine. In this assay, a lower detection limit of l-histidine (50 nM) is addressed comparing to previously reported colorimetric methods. The cost is extremely low as the proposed design is both label-free and enzyme-free. All the more vitally, the colorimetric detection procedure is substantially straightforward with no further modification processes. By and large, the sensor can provide a promising plan for the detection of l-histidine.
ISSN:1873-3557