Remodeling of the HDL in NIDDM: a fundamental role for cholesteryl ester transfer protein

• Published in: American journal of physiology: endocrinology and metabolism Vol. 274; no. 6; p. E1091
• Main Authors: Castle, Christine K, Kuiper, Susan L, Blake, William L, Paigen, Beverly, Marotti, Keith R, Melchior, George W
• Format: Journal Article
• Language: English
• Published: United States 01.06.1998
• Subjects: high-density lipoprotein lipase; KKAy mouse; free apolipoprotein A-I; pre-β apolipoprotein A-I; mouse model of non-insulin-dependent diabetes mellitus; mouse plasma lipase
• ISSN: 1522-1555

When the A gene is expressed in KK mice, the yellow offspring (KKA mice) become obese, insulin resistant, hyperglycemic, and severely hypertriglyceridemic, yet they maintain extraordinarily high plasma high-density lipoprotein (HDL) levels. Mice lack the ability to redistribute neutral lipids among circulating lipoproteins, a process catalyzed in humans by cholesteryl ester transfer protein (CETP). To test the hypothesis that it is the absence of CETP that allows these hypertriglyceridemic mice to maintain high plasma HDL levels, simian CETP was expressed in the KKA mouse. The KKA -CETP mice retained the principal characteristics of KKA mice except that their plasma HDL levels were reduced (from 159 ± 25 to 25 ± 6 mg/dl) and their free apolipoprotein A-I concentrations increased (from 7 ± 3 to 22 ± 6 mg/dl). These changes appeared to result from a CETP-induced enrichment of the HDL with triglyceride (from 6 ± 2 to 60 ± 18 mol of triglyceride/mol of HDL), an alteration that renders HDL susceptible to destruction by lipases. These data support the premise that CETP-mediated remodeling of the HDL is responsible for the low levels of that lipoprotein that accompany hypertriglyceridemic non-insulin-dependent diabetes mellitus.