Corin protects H 2 O 2 -induced apoptosis through PI3K/AKT and NF-κB pathway in cardiomyocytes
The functional role of corin in H O -induced apoptosis is largely unexplored. The present study investigated the protective role of corin against cell injury by possible involvement of PI3K/AKT and NF-kB signaling pathways in cardiomyocytes. Cardiomyocytes H9c2 and HL-1 cells were used in the study....
Saved in:
Published in | Biomedicine & pharmacotherapy Vol. 97; p. 594 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
France
01.01.2018
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The functional role of corin in H
O
-induced apoptosis is largely unexplored. The present study investigated the protective role of corin against cell injury by possible involvement of PI3K/AKT and NF-kB signaling pathways in cardiomyocytes.
Cardiomyocytes H9c2 and HL-1 cells were used in the study. Cell viability was measured using CCK-8 assay; cell apoptosis was analyzed by flow cytometry, TUNEL assay, and western blot; and cell migration was measured using wound healing assay. The fluorescent intensities of reactive oxygen species (ROS) were measured using a flow cytometer. Quantitative RT-PCR was used to measure the mRNA expression of corin. Western blot was used to measure the protein expression of corin, apoptosis-related proteins (Bax, cleaved-Caspase-3 and -9), and PI3K/AKT and NF-κB signaling pathway proteins.
Treatment with H
O
(150μM, 6h) significantly decreased cell viability and relative migration, increased apoptosis, and decreased the expression of corin in H9c2 and HL-1 cells. Overexpression of corin alleviated the H
O
-induced cell injury by increasing cell viability and migration and decreasing apoptosis in the cardiomyocytes. Overexpression of corin also decreased the ROS level in the cardiomyocytes likely through upregulating HIF-1α. These effects of corin on the cell injury might be mediated via the corin-induced activations of PI3K/AKT and NF-κB signaling pathways.
Overexpression of corin protected cardiomyocytes from H
O
-induced injury by decreasing apoptosis and ROS level via activations of the PI3K/AKT and NF-κB signaling pathways and upregulating HIF-1α. |
---|---|
ISSN: | 1950-6007 |