Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building [Short communication]

The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-process...

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Published inThe international journal of tuberculosis and lung disease Vol. 16; no. 11; p. 1535
Main Authors David, S, Sutre, A F, Sanca, A, Mané, A, Henriques, V, Portugal, C, Sancho, L, Cardoso, A, Paixão, E, Duarte, E L, Leite, C Q F, Salem, J I, Antunes, A
Format Journal Article
LanguageEnglish
Published France 01.11.2012
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Summary:The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.
ISSN:1815-7920