Effect of sulfated galactan from Porphyra haitanensis on H 2 O 2 -induced premature senescence in WI-38 cells

Porphyran sulfated galactan extracted from red algae Porphyra haitanensis is a sulfated polysaccharide, which possesses excellent activities. In the present study, WI-38 cells were treated with H O to induce premature senescence and then the protection of porphyran against aging in vitro and associa...

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Published inInternational journal of biological macromolecules Vol. 106; p. 1235
Main Authors Zhang, Zhongshan, Wang, Xiaomei, Su, Huili, Pan, Yongliang, Han, Jianfang, Zhang, Taisheng, Mao, Genxiang
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.2018
Subjects
Antioxidants - chemistry
Antioxidants - therapeutic use
Cell Line
Cellular Senescence - drug effects
Galactans - chemistry
Galactans - therapeutic use
Humans
Hydrogen Peroxide - toxicity
Polysaccharides - chemistry
Polysaccharides - therapeutic use
Porphyra - chemistry
Sulfates - chemistry
Cellular senescence
Antioxidant activities
Sulfated galactan
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Summary:Porphyran sulfated galactan extracted from red algae Porphyra haitanensis is a sulfated polysaccharide, which possesses excellent activities. In the present study, WI-38 cells were treated with H O to induce premature senescence and then the protection of porphyran against aging in vitro and associated molecular mechanisms were investigated. The protection occurred in a dose-dependent manner, offering an optimal efficacy starting at 10μg/mL. The proportion of SA-β-gal positive cells in porphyran group decreases from 53% to 23% in the cultures at 30 PDs. Porphyran has been detected specifically reducing SAHF-like foci formation in senescent cells. In addition, porphyran significantly affected the p53-p21 pathways in H O -treated WI-38 cells. Our data suggest the promising role of porphyran as an attractive and bio-safe agent with the potential to retard senescence and attenuate senescence-related diseases.
ISSN:1879-0003