Determination of serum aflatoxin B 1 -lysine to evaluate the efficacy of an aflatoxin-adsorbing feed additive in pigs fed an aflatoxin B 1 -contaminated diet

• Published in: Mycotoxin research Vol. 33; no. 2; p. 93
• Main Authors: Di Gregorio, Mayra Carraro, Jager, Alessandra Vincenzi, Souto, Pollyana Cristina Maggio Castro, Costa, Aline Alves, Rottinghaus, George Edwin, Passarelli, Danielle, Budiño, Fabio Enrique Lemos, Corassin, Carlos Humberto, Oliveira, Carlos Augusto Fernandes
• Format: Journal Article
• Language: English
• Published: Germany 01.05.2017
• Subjects: Adsorption; Aflatoxin B1 - blood; Aflatoxin B1 - isolation & purification; Aluminum Silicates; Animal Feed; Animals; Diet - methods; Food Additives; Food Contamination; Lysine - blood; Mycotoxins - blood; Mycotoxins - isolation & purification; Serum - chemistry; Swine; LC-MS/MS; Swine; AFB1-lysine adduct; Adsorbent
• ISSN: 1867-1632

In this study, serum aflatoxin B (AFB )-lysine was determined in order to evaluate the in vivo efficacy of a hydrated sodium calcium aluminosilicate (HSCAS) in pigs fed AFB . Twenty-four 49-day-old crossbred barrows were maintained in individual cages and allowed ad libitum access to feed and water. A completely randomized design was used with six animals assigned to each of four dietary treatments for 21 days as follows: (A) basal diet (BD), (B) BD supplemented with 0.5 % HSCAS, (C) BD supplemented with 1.1 mg/kg AFB , and (D) BD supplemented with 0.5 % HSCAS and 1.1 mg/kg AFB . HSCAS was able to alleviate the toxic effects of AFB on pigs and reduce (P < 0.05) the levels of serum AFB -lysine. Cumulative reductions of adduct yield values, calculated through the equation [(pg AFB -lysine/mg albumin) / (μg AFB /kg body weight)], were 53.0, 62.8, and 72.1 after 7, 14, and 21 days of oral exposure, respectively. AFB -lysine has potential as an AFB -specific biomarker for diagnostic purposes and for evaluating the efficacy of chemoprotective interventions in pigs.