Genome sequence and overview of Oligoflexus tunisiensis Shr3 T in the eighth class Oligoflexia of the phylum Proteobacteria

• Published in: Standards in genomic sciences Vol. 11; p. 90
• Main Authors: Nakai, Ryosuke, Fujisawa, Takatomo, Nakamura, Yasukazu, Baba, Tomoya, Nishijima, Miyuki, Karray, Fatma, Sayadi, Sami, Isoda, Hiroko, Naganuma, Takeshi, Niki, Hironori
• Format: Journal Article
• Language: English
• Published: England 2016
• Subjects: Proteobacteria; RND-type efflux pump; Denitrification; Nitrous oxide (N2O); Oligoflexia
• ISSN: 1944-3277; 1944-3277

Shr3 is the first strain described in the newest (eighth) class of the phylum . This strain was isolated from the 0.2-μm filtrate of a suspension of sand gravels collected in the Sahara Desert in the Republic of Tunisia. The genome of Shr3 is 7,569,109 bp long and consists of one scaffold with a 54.3% G + C content. A total of 6,463 genes were predicted, comprising 6,406 protein-coding and 57 RNA genes. Genome sequence analysis suggested that strain Shr3 had multiple terminal oxidases for aerobic respiration and various transporters, including the resistance-nodulation-cell division-type efflux pumps. Additionally, gene sequences related to the incomplete denitrification pathway lacking the final step to reduce nitrous oxide (N O) to nitrogen gas (N ) were found in the Shr3 genome. The results presented herein provide insight into the metabolic versatility and N O-producing activity of species.