The Draft Genome Sequence of Actinokineospora bangkokensis 44EHW T Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units

We report the draft genome sequence of 44EHW , the producer of the antifungal polyene compounds, thailandins A and B. The sequence contains 7.45 Mb, 74.1% GC content and 35 putative gene clusters for the biosynthesis of secondary metabolites. There are three gene clusters encoding large polyketide s...

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Bibliographic Details
Published inMolecules (Basel, Switzerland) Vol. 21; no. 11
Main Authors Greule, Anja, Intra, Bungonsiri, Flemming, Stephan, Rommel, Marcel G E, Panbangred, Watanalai, Bechthold, Andreas
Format Journal Article
LanguageEnglish
Published Switzerland 23.11.2016
Subjects
Actinobacteria - genetics
Actinobacteria - metabolism
Antifungal Agents - metabolism
Base Composition
Base Sequence
Biosynthetic Pathways
Chromosome Mapping
Genome Size
Genome, Bacterial
Multigene Family
Actinokineospora bangkokensis
draft genome sequence
thailandin
biosynthetic gene cluster
butylmalonyl-CoA
genome mining
polyene
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Summary:We report the draft genome sequence of 44EHW , the producer of the antifungal polyene compounds, thailandins A and B. The sequence contains 7.45 Mb, 74.1% GC content and 35 putative gene clusters for the biosynthesis of secondary metabolites. There are three gene clusters encoding large polyketide synthases of type I. Annotation of the ORF functions and targeted gene disruption enabled us to identify the cluster for thailandin biosynthesis. We propose a plausible biosynthetic pathway for thailandin, where the unusual butylmalonyl-CoA extender unit is incorporated and results in an untypical side chain.
ISSN:1420-3049