The meconium antigen exhibiting oncofetal properties

A rabbit antiserum to the meconium antigen of the human fetal intestine is obtained by immunization of rabbits by this substance preparation. The preparation is extracted from meconium by the sulphate ammonium (70% saturation) treatment and gel filtration of supernatant with selection of the 240 kD...

Full description

Saved in:
Bibliographic Details
Published inEksperimentalʹnaja onkologija Vol. 11; no. 4; p. 47
Main Authors Pugachev, K K, Shimbireva, I B, Avdeev, G I
Format Journal Article
LanguageRussian
Published Ukraine 1989
Subjects
Absorption
Animals
Antibody Specificity
Antigens - analysis
Antigens - isolation & purification
Antigens, Neoplasm - analysis
Antigens, Neoplasm - isolation & purification
Chromatography, Gel
Colon - immunology
Colonic Neoplasms - immunology
Electrophoresis, Polyacrylamide Gel
Epithelium - immunology
Humans
Immune Sera - analysis
Immune Sera - isolation & purification
Immunization
Immunoenzyme Techniques
Infant, Newborn
Meconium - immunology
Rabbits
Online AccessGet more information

Cover

More Information
Summary:A rabbit antiserum to the meconium antigen of the human fetal intestine is obtained by immunization of rabbits by this substance preparation. The preparation is extracted from meconium by the sulphate ammonium (70% saturation) treatment and gel filtration of supernatant with selection of the 240 kD fractions. In the indirect immunoperoxidase reaction the antiserum reacts with epithelium of certain normal and fetal tissues. It displays oncofetal features in colon, since it reacts with fetal and malignant epithelium and does not react with normal epithelium of this organ. The developed test-system may be used in pathomorphology for the morphological and functional state of colon mucosa evaluation.
ISSN:0204-3564