Antihypertensive Effects of Artemisia scoparia Waldst in Spontaneously Hypertensive Rats and Identification of Angiotensin I Converting Enzyme Inhibitors

• Published in: Molecules (Basel, Switzerland) Vol. 20; no. 11; p. 19789
• Main Authors: Cho, Jeong-Yong, Park, Kyung-Hee, Hwang, Do Young, Chanmuang, Saoraya, Jaiswal, Lily, Park, Yang-Kyun, Park, Sun-Young, Kim, So-Young, Kim, Haeng-Ran, Moon, Jae-Hak, Ham, Kyung-Sik
• Format: Journal Article
• Language: English
• Published: Switzerland 03.11.2015
• Subjects: Angiotensin-Converting Enzyme Inhibitors - chemistry; Angiotensin-Converting Enzyme Inhibitors - isolation & purification; Angiotensin-Converting Enzyme Inhibitors - pharmacology; Animals; Antihypertensive Agents - chemistry; Antihypertensive Agents - isolation & purification; Antihypertensive Agents - pharmacology; Artemisia - chemistry; Blood Pressure - drug effects; Gene Expression Regulation - drug effects; Hypertension - drug therapy; Hypertension - metabolism; Hypertension - physiopathology; Molecular Structure; Nitric Oxide - metabolism; Nuclear Magnetic Resonance, Biomolecular; Oxidative Stress - drug effects; Peptidyl-Dipeptidase A - blood; Peptidyl-Dipeptidase A - metabolism; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Rats; Rats, Inbred SHR; rhoA GTP-Binding Protein - genetics; rhoA GTP-Binding Protein - metabolism; Vascular Endothelial Growth Factor A - genetics; Vascular Endothelial Growth Factor A - metabolism; blood pressure; halophyte; ACE inhibitor; Artemisia scoparia; phenolics
• ISSN: 1420-3049

We investigated the antihypertensive effects of Artemisia scoparia (AS) in spontaneously hypertensive rats (SHR). The rats were fed diets containing 2% (w/w) hot water extracts of AS aerial parts for 6 weeks. The AS group had significantly lower systolic and diastolic blood pressure levels than the control group. The AS group also had lower angiotensin I converting enzyme (ACE) activity and angiotensin II content in serum compared to the control group. The AS group showed higher vascular endothelial growth factor and lower ras homolog gene family member A expression levels in kidney compared to the control group. The AS group had significantly lower levels of plasma lipid oxidation and protein carbonyls than the control group. One new and six known compounds were isolated from AS by guided purification. The new compound was determined to be 4'-O-β-D-glucopyranoyl (E)-4-hydroxy-3-methylbut-2-enyl benzoate, based on its nuclear magnetic resonance and electrospray ionization-mass spectroscopy data.