Analysis of FHIT gene methylation in egyptian breast cancer women: association with clinicopathological features

• Published in: Asian Pacific journal of cancer prevention : APJCP Vol. 16; no. 3; p. 1235
• Main Authors: Zaki, Seham Mahrous, Abdel-Azeez, Hala A, El Nagar, Mona Roshdy, Metwally, Khaled Abdel-Aziz, S Ahmed, Marwa M Samir
• Format: Journal Article
• Language: English
• Published: Thailand 2015
• Subjects: Acid Anhydride Hydrolases - genetics; Adult; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Carcinoma, Ductal, Breast - genetics; Carcinoma, Ductal, Breast - secondary; Carcinoma, Lobular - genetics; Carcinoma, Lobular - secondary; Case-Control Studies; DNA Methylation; Egypt; Female; Follow-Up Studies; Gene Expression Regulation, Neoplastic; Humans; Lymphatic Metastasis; Middle Aged; Neoplasm Grading; Neoplasm Proteins - genetics; Neoplasm Staging; Polymerase Chain Reaction; Prognosis; Promoter Regions, Genetic - genetics; Egypt
• ISSN: 2476-762X

Fragile histidine triad (FHIT) gene is a tumor suppressor gene which involved in breast cancer pathogenesis. Epigenetics alterations in FHIT contributes to tumorigenesis of breast cancer. Our objective was to study FHIT promoter region hypermethylation in Egyptian breast cancer patients and its association with clinicopathological features. Methylation-specific polymerase chain reaction was performed to study the hypermethylation of FHIT promoter region in 20 benign breast tissues and 30 breast cancer tissues. The frequency of hypermethylation of FHIT promoter region was significantly increased in breast cancer patients compared to bengin breast disease patients. The Odd ?s ratio (95%CI) of development of breast cancer in individuals with FHIT promoter hypermethylation (MM) was 11.0 (1.22-250.8). There were also significant associations between FHIT promoter hypermethylation and estrogen, progesterone receptors negativity, tumor stage and nodal involvment in breast cancer pateints. Our results support an association between FHIT promotor hypermethylation and development of breast cancer in Egyptian breast cancer patients. FHIT promoter hypermethylation is associated with some poor prognostic features of breast cancer.