Sensitive determination of four β 2 -agonists in pig feed by capillary electrophoresis using on-line sample preconcentration with contactless conductivity detection
In this work, an on-line preconcentration method of field-enhanced sample injection (FESI) was implemented in the determination of four β -agoinsts terbutaline (TER), procaterol (PRO), formoterol (FOR) and bambuterol (BAM) by capillary electrophoresis coupled with capacitively coupled contactless co...
Saved in:
Published in | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 973C; p. 29 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
15.12.2014
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | In this work, an on-line preconcentration method of field-enhanced sample injection (FESI) was implemented in the determination of four β
-agoinsts terbutaline (TER), procaterol (PRO), formoterol (FOR) and bambuterol (BAM) by capillary electrophoresis coupled with capacitively coupled contactless conductivity detection (CE-C
D). Under optimized conditions the background electrolyte (BGE) was 5mM Tris(hydroxymethyl)aminomethane (Tris) and 10mM citric acid (Cit) at a pH of 3.2 while the sample dilution solution was obtained by methanol. The detection limits (defined as S/N=3) of this method were 0.02mg/L for TER, PRO, FOR, BAM, which were much lower than that of the conventional CE-C
D method without preconcentration procedure, the enhancement factors were greatly improved to be 30-40-fold. The linearity ranges of four β
-agoinsts were 0.1-15mg/L, with good linear correlation coefficients (r
>0.9900). In order to evaluate the application potential of the developed method, real sample from pig feed was analyzed with recoveries of 91.4-106.2%. |
---|---|
ISSN: | 1873-376X |