Coffee (Coffea arabica L.)

Coffee (Coffea sp.) is a perennial plant widely cultivated in many tropical countries. It is a cash crop for millions of small farmers in these areas. As for other tree species, coffee has long breeding cycles, which makes conventional breeding programs time-consuming. For that matter, genetic trans...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1224; p. 275
Main Authors Déchamp, Eveline, Breitler, Jean-Christophe, Leroy, Thierry, Etienne, Hervé
Format Journal Article
LanguageEnglish
Published United States 2015
Subjects
Agrobacterium tumefaciens - genetics
Agrobacterium tumefaciens - growth & development
Coculture Techniques
Coffea - genetics
Coffea - growth & development
Coffea - physiology
Genetic Engineering - methods
Plant Leaves - growth & development
Plant Roots - growth & development
Plants, Genetically Modified
Regeneration
Seeds - growth & development
Sterilization
Transformation, Genetic
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Summary:Coffee (Coffea sp.) is a perennial plant widely cultivated in many tropical countries. It is a cash crop for millions of small farmers in these areas. As for other tree species, coffee has long breeding cycles, which makes conventional breeding programs time-consuming. For that matter, genetic transformation can be an effective way to introduce a desired trait in elite varieties or for functional genomics. In this chapter, we describe two highly efficient and reliable Agrobacterium-mediated transformation techniques developed for the C. arabica cultivated species: (1) A. tumefaciens to study and introduce genes conferring resistance/tolerance to biotic (coffee leaf rust, insects) and abiotic stress (drought, heat, seed desiccation) in fully transformed plants and (2) A. rhizogenes to study candidate gene expression for nematode resistance in transformed roots.
ISSN:1940-6029