Inhibition of lung tumor growth in nude mice by siRNA CD31 targeting PECAM-1

Small interfering RNA (siRNA) provides a promising therapeutic approach in the silencing of disease-causing genes. In the present study, the use of 2'-O-methyl-modified siRNA-cluster of differentiation 31 (siRNA ), with cationic liposome RNA interference (RNAi)-mate as a carrier, effectively si...

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Published inOncology letters Vol. 8; no. 1; p. 33
Main Authors Ouyang, Jin-Sheng, Li, Yu-Ping, Chen, Cheng-Shui, Chen, Jun-Jie, Chen, Tong-Ke, Cai, Chang, Yang, Li
Format Journal Article
LanguageEnglish
Published Greece 01.07.2014
Subjects
carcinoma
vascular endothelial growth factor
platelet endothelial adhesion molecule
small interfering RNA
tumor microenvironment
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Summary:Small interfering RNA (siRNA) provides a promising therapeutic approach in the silencing of disease-causing genes. In the present study, the use of 2'-O-methyl-modified siRNA-cluster of differentiation 31 (siRNA ), with cationic liposome RNA interference (RNAi)-mate as a carrier, effectively silenced the platelet endothelial cell molecule 1 (PECAM-1) gene of murine hemangioendothelioma cells , 2'-O-methyl-modified siRNA carried by RNAi-mate was successfully delivered, targeting the PECAM-1 gene in the vasculature of nude mouse lung carcinoma xenografts. The growth of the lung carcinoma xenografts was inhibited by the 2'-O-methyl-modified siRNA and RNAi-mate complexes, and the expression of the PECAM-1 protein was downregulated, with a simultaneous decrease in vascular endothelial growth factor (VEGF) protein in the lung carcinoma xenografts. 2'-O-methyl-modified siRNA -RNAi-mate complexes may provide a potential therapeutic strategy in lung carcinoma treatment. The effect of PECAM-1 on VEGF expression may possibly be attributed to the function of PECAM-1 signal transduction.
ISSN:1792-1074