Antitoxic and antioxidant effects of N-stearoylethanolamin in the content of nanocomposite complex with doxorubicin in organs of mice with Lewis carcinoma

• Published in: Ukrains'kyi biokhimichnyi zhurnal (1999 ) Vol. 85; no. 5; p. 97
• Main Authors: Hudz', Ie A, Hula, N M, Horid'ko, T M, Bashta, Iu M, Voieĭkov, A I, Berdyshev, A H, Kosiakova, H V, Panchuk, R R, Stoĭka, R S, Riabtseva, A O, Zaichenko, O S
• Format: Journal Article
• Language: Ukrainian
• Published: Ukraine 01.09.2013
• Subjects: Alanine Transaminase - blood; Animals; Antibiotics, Antineoplastic - chemistry; Antibiotics, Antineoplastic - pharmacology; Antioxidants - chemistry; Antioxidants - pharmacology; Aspartate Aminotransferases - blood; Carcinoma, Lewis Lung - drug therapy; Carcinoma, Lewis Lung - metabolism; Catalase - antagonists & inhibitors; Catalase - metabolism; Creatinine - blood; Doxorubicin - chemistry; Doxorubicin - pharmacology; Ethanolamines - chemistry; Ethanolamines - pharmacology; Glutathione Peroxidase - metabolism; Heart - drug effects; Kidney - drug effects; Kidney - metabolism; Lipid Peroxidation - drug effects; Liver - drug effects; Liver - metabolism; Male; Mice; Mice, Inbred C57BL; Nanocomposites; Polyethylene Glycols - chemistry; Stearic Acids - chemistry; Stearic Acids - pharmacology; Superoxide Dismutase - antagonists & inhibitors; Superoxide Dismutase - metabolism

The aim of the study was to evaluate the possibility to reduce the doxorubicin toxic effects by its immobilization with N-stearoylethanolamine (NSE) on nanocarier polyethylene glycol. The studied parameters of the doxorubicin toxicity were: the level of creatinine in the mice blood plasma and activity of alanine aminotransferase and aspartate aminotransferase in the blood plasma of mice. The activity of catalase superoxide dismutase, glutathione peroxidase and intensity of lipid peroxidation was determined in the tissues of the heart, kidneys and liver. Doxorubicin in the content of nanocarrier alone caused an increase of serum creatinine and aspartateaminotrasferase activity in plasma of experimental animals with carcinoma. Nanocomposite which contained doxorubicin and NSE, did not cause an increase of these parameters. It has been shown that the administration of a carrier containing doxorubicin to mice with Lewis lung carcinoma caused the decrease of catalase activity in mice with carcinoma. The combination of NSE and doxorubicin on the carrier led to the normalization of this parameter to the level of intact animals. NSE immobilized on a carrier together with doxorubicin caused a decrease in the activity of superoxide dismutase in the kidney tissue of mice with tumor. The tumor growth caused the increase of the of superoxide dismutase in mice. The administration of a carrier which contained doxorubicin and NSE normalized superoxide dismutase in heart tissue contrary of kidney. The obtained results show the antitoxic and antioxidant effects of N-stearoylethanolamine immobilized in the nanocarrier complex together with doxorubicin.