In vivo microdialysis sampling coupled to liquid chromatography for the study of acetaminophen metabolism

In vivo microdialysis sampling coupled to liquid chromatography is a powerful tool for the study of drug metabolism. This technique is illustrated by investigating the pharmacokinetics of acetaminophen in the blood and liver of an anesthetized rat. The pharmacokinetics of the sulfate and glucuronide...

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Bibliographic Details
Published inJournal of chromatography Vol. 506; p. 461
Main Authors Scott, D O, Sorensen, L R, Lunte, C E
Format Journal Article
LanguageEnglish
Published Netherlands 11.05.1990
Subjects
Acetaminophen - pharmacokinetics
Animals
Chromatography, Liquid
Dialysis
Glucuronates - metabolism
Liver - metabolism
Microchemistry
Rats
Rats, Inbred Strains
Sulfates - metabolism
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Summary:In vivo microdialysis sampling coupled to liquid chromatography is a powerful tool for the study of drug metabolism. This technique is illustrated by investigating the pharmacokinetics of acetaminophen in the blood and liver of an anesthetized rat. The pharmacokinetics of the sulfate and glucuronide metabolites as well as the parent acetaminophen can be determined with high precision using microdialysis sampling. Microdialysis samples can be collected at a high rate from several sites without fluid loss with a single animal. Because the animal serves as its own control better data can be obtained. Liquid chromatography provides determination of multiple analytes per sample for metabolic profiling. This technique will provide more accurate and precise pharmacokinetic data while requiring fewer animals.