Identification of metabolites of honokiol in rat urine using 13C stable isotope labeling and liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry

A general approach based on stable isotope labeling and UPLC/Q-TOF-MS analysis of in vivo novel metabolites of honokiol has been developed in our study. In this method, urine samples were collected after intravenous administration of mixture of regular and [(13)C6]-labeled honokiol at 1:1 ratio to h...

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Published inJournal of chromatography. A Vol. 1295; p. 48
Main Authors Liu, Juan, Tang, Minghai, Lai, Huijun, Dong, Yinfeng, Xie, Caifeng, Ye, Haoyu, Ma, Liang, Qiu, Neng, Li, Yanfang, Cai, Lulu, Chen, Lijuan
Format Journal Article
LanguageEnglish
Published Netherlands 21.06.2013
Subjects
Animals
Biotransformation
Biphenyl Compounds - chemistry
Biphenyl Compounds - metabolism
Chromatography, Liquid - methods
Drugs, Chinese Herbal - chemistry
Drugs, Chinese Herbal - metabolism
Isotope Labeling
Lignans - chemistry
Lignans - metabolism
Magnolia - chemistry
Male
Rats
Rats, Wistar
Tandem Mass Spectrometry - methods
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Summary:A general approach based on stable isotope labeling and UPLC/Q-TOF-MS analysis of in vivo novel metabolites of honokiol has been developed in our study. In this method, urine samples were collected after intravenous administration of mixture of regular and [(13)C6]-labeled honokiol at 1:1 ratio to healthy rats. The metabolites could be easily recognized by the determination of a chromatographically co-eluted pair of isotopomers (MS doublet peaks) with similar peak intensities and mass difference corresponding to that between isotope-labeled and non-isotope-labeled honokiol. A total of 51 metabolites were detected, 37 of which were tentatively identified based on mass accuracy (<5 ppm). Among them, 33 of honokiol metabolites were first reported with 5 metabolites belonging to phase I and other 32 metabolites belonging to phase II metabolites. Our results highlighted that the main phase I metabolic pathways of honokiol in rats were oxidation, and the phase II metabolic pathways were sulfation, glucuronidation, acetylation as well as amino acids conjugation. This was the first research focused on the biotransformation of honokiol in rats, and the identification of these metabolites might provide us essential information for further pharmacological and clinical studies of honokiol.
ISSN:1873-3778