Surface CD3 expression proceeds through both myosin regulatory light chain 9 (MYL9)-dependent and MYL9-independent pathways in Jurkat cells

• Published in: Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi Vol. 48; no. 5-6; p. 137
• Main Authors: Aoki, Takumi, Miyazaki, Koji, Katayama, Takuji, Watanabe, Mariko, Horie, Ryoichi, Danbara, Mikio, Higashihara, Masaaki
• Format: Journal Article
• Language: English
• Published: Japan 2012
• Subjects: Carcinogens - pharmacology; CD3 Complex - biosynthesis; CD3 Complex - genetics; Gene Expression Regulation - drug effects; Gene Expression Regulation - physiology; Humans; Jurkat Cells; Myosin Light Chains - biosynthesis; Myosin Light Chains - genetics; Protein Kinase C - genetics; Protein Kinase C - metabolism; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Signal Transduction - drug effects; Signal Transduction - physiology; Tetradecanoylphorbol Acetate - pharmacology
• ISSN: 1884-8796

CD3 is a complex of polypeptides which form part of the T cell receptor. Normal human peripheral pan T cells, express not only CD3, but the mRNA for myosin regulatory light chains MYL9, MYL12A, and MYL12B are also significantly expressed. In the Jurkat wild strain, an acute T cell leukemia cell line, CD3 on the surface and MYL9 mRNA are not expressed, while both MYL12A mRNA and MYL12B mRNA are expressed. Jurkat-I, a new clone was established by the transfection of the MYL9 gene into the Jurkat wild strain. As a result, the level of CD3 expressed on the surface of Jurkat-I cells was significantly higher than those in the Jurkat wild strain. Phorbol 12-myristate 13-acetate increased the surface CD3 levels in Jurkat wild strain cells without resulting in MYL9 gene expression, indicating that protein kinase C is partially involved in the expression of CD3 on the surface. These results suggest that surface CD3 expression proceeds through both MYL9-dependent and MYL9-independent pathways (i.e. the protein kinase C- dependent pathway) in Jurkat cells.