The effect of potential-dependent potassium uptake on membrane potential in rat brain mitochondria

The effect of potential-dependent potassium uptake on the transmembrane potential difference (DeltaPsi(m)) in rat brain mitochondria has been studied. It was shown that in potassium concentration range of 0-120 mM the potential-dependent K(+)-uptake into matrix leads to the increase in respiration r...

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Published inUkrains'kyi biokhimichnyi zhurnal (1999 ) Vol. 85; no. 1; p. 33
Main Authors Akopova, O V, Nosar', V I, Kolchinskaia, L I, Man'kovskaia, I N, Malysheva, M K, Sagach, V F
Format Journal Article
LanguageRussian
Published Ukraine 01.01.2013
Subjects
Adenosine Triphosphate - metabolism
Animals
Brain - drug effects
Brain - metabolism
Culture Media
Decanoic Acids - pharmacology
Glyburide - pharmacology
Hydroxy Acids - pharmacology
Ion Transport - drug effects
Kinetics
Membrane Potential, Mitochondrial - drug effects
Mitochondria - drug effects
Mitochondria - metabolism
Oxidative Phosphorylation - drug effects
Potassium - metabolism
Potassium - pharmacology
Potassium Channel Blockers - pharmacology
Potassium Channels - metabolism
Rats
Rats, Wistar
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Summary:The effect of potential-dependent potassium uptake on the transmembrane potential difference (DeltaPsi(m)) in rat brain mitochondria has been studied. It was shown that in potassium concentration range of 0-120 mM the potential-dependent K(+)-uptake into matrix leads to the increase in respiration rate and mitochondrial depolarization. ATP-dependent potassium channel (K+(ATP)-channel) blockers, glibenclamide and 5-hydroxydecanoate, block approximately 35% of potential-dependent potassium uptake in the brain mitochondria. It was shown that K+(ATP)-channel blockage results in membrane repolarization by approximately 20% of control, which is consistent with experimental dependence of DeltaPsi(m) on the rate of potential-dependent potassium uptake. Obtained experimental data give the evidence that functional activity of K+(ATP)-channel is physiologically important in the regulation of membrane potential and energy-dependent processes in brain mitochondria.