Effect of N-stearoylethanolamine on the DNA fragmentation intensity in tumour and extratumoral tissues of the human adrenal cortex

• Published in: Ukrains'kyi biokhimichnyi zhurnal (1999 ) Vol. 84; no. 4; p. 49
• Main Authors: Levchuk, N I, Pushkar'ov, V M, Kovzun, O I, Mykosha, O S, Hula, N M, Tron'ko, M D
• Format: Journal Article
• Language: Ukrainian
• Published: Ukraine 01.07.2012
• Subjects: Adrenal Cortex Hormones - metabolism; Adrenal Gland Neoplasms - drug therapy; Adrenal Gland Neoplasms - metabolism; Adrenal Gland Neoplasms - pathology; Adrenal Glands - drug effects; Adrenal Glands - metabolism; Adrenal Glands - pathology; Antineoplastic Agents - pharmacology; Antioxidants - pharmacology; Cushing Syndrome - drug therapy; Cushing Syndrome - metabolism; Cushing Syndrome - pathology; DNA Fragmentation - drug effects; Drug Resistance, Neoplasm; Ethanolamines - pharmacology; Humans; Hyperplasia - drug therapy; Hyperplasia - metabolism; Hyperplasia - pathology; Microtomy; Neoplasms - drug therapy; Neoplasms - metabolism; Neoplasms - pathology; Organ Specificity; Stearic Acids - pharmacology; Tissue Culture Techniques; Tumor Microenvironment

The effect of different concentrations of N-stearoylethanolamine (NSE 18:0) on fragmentation of DNA in the tumoural and extratumour tissues of the adrenal glands in vitro was studied. In this work the following types of tissue were investigated: extratumoural tissue from patients with hormonally active tumours, benign tumour tissue (hormonally active and hormonally inactive), tissue of malignant tumours and hyperplasic tissue of the adrenal glands (Itsenko-Cushing disease). It has been established that the NSE increases the intensity of DNA fragmentation only in the tissue of hormonally inactive tumours. Benign hormonally active tumours, malignant tumours and hyperplastic tissue of the adrenal glands were resistant to the NSE. The possible mechanisms of resistance to the drug are discussed.