The analysis of biochemical markers of MCF-7 cells apoptosis induced by recombinant analog of lactaptin

Earlier we isolated and characterized human milk pro-apoptotic peptide - lactaptin and generated its engineered analog - RL2. It was shown that both lactaptin and RL2 are capable to induce apoptotic death of MCF-7 cells. In this report we have analyzed biochemical markers of RL2 induced MCF-7 apopto...

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Published inBioorganicheskaia khimiia Vol. 38; no. 1; p. 92
Main Authors Fomin, A S, Koval', O A, Semenov, D V, Potapenko, M O, Kuligina, E V, Kit, Iu Ia, Rikhter, V A
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.2012
Subjects
Apoptosis - drug effects
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Breast Neoplasms - drug therapy
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Caseins - genetics
Caseins - pharmacology
Caspases - metabolism
Cell Line, Tumor
Female
Humans
Membrane Potential, Mitochondrial - drug effects
Protein Engineering
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Summary:Earlier we isolated and characterized human milk pro-apoptotic peptide - lactaptin and generated its engineered analog - RL2. It was shown that both lactaptin and RL2 are capable to induce apoptotic death of MCF-7 cells. In this report we have analyzed biochemical markers of RL2 induced MCF-7 apoptosis. The activation of initiator and effector caspases as well as mitochondrial membrane potential and cytoplasm membrane changes were analyzed using flow cytometry and Western-blot methods. We have found that RL2 induced apoptotic death of MCF-7 cells was accompanied by PS exposure on the plasma membrane surface. It also was shown that RL2 has induced dissipation of mitochondrial membrane potential and resulted in activation of initiator caspases 8, 9 and effector caspase 7.
ISSN:0132-3423