Astroglia proteins in the rat brain in experimental chronic hepatitis and 2-oxoglutarate effect

A probable increase of the level of calcium-binding protein S100b and soluble form of glial fibrillary acidic protein (GFAP), as well as reducing the level of filament GFAP in the brain of Wistar rats under hepatic encephalopathy development caused by chronic hepatitis (HP) were shown. Increasing co...

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Bibliographic Details
Published inUkrains'kyi biokhimichnyi zhurnal (1999 ) Vol. 83; no. 1; p. 69
Main Authors Fomenko, O Z, Ushakova, H O, Piierzhynovs'kyĭ, S H
Format Journal Article
LanguageUkrainian
Published Ukraine 01.01.2011
Subjects
Animals
Astrocytes - metabolism
Brain - metabolism
Cytoskeleton - metabolism
Cytosol - metabolism
Electrophoresis, Polyacrylamide Gel
Glial Fibrillary Acidic Protein - metabolism
Hepatic Encephalopathy - etiology
Hepatic Encephalopathy - metabolism
Hepatic Encephalopathy - prevention & control
Hepatitis, Chronic - complications
Hepatitis, Chronic - metabolism
Immunoblotting
Ketoglutaric Acids - administration & dosage
Ketoglutaric Acids - therapeutic use
Nerve Growth Factors - metabolism
Rats
Rats, Wistar
S100 Calcium Binding Protein beta Subunit
S100 Proteins - metabolism
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Summary:A probable increase of the level of calcium-binding protein S100b and soluble form of glial fibrillary acidic protein (GFAP), as well as reducing the level of filament GFAP in the brain of Wistar rats under hepatic encephalopathy development caused by chronic hepatitis (HP) were shown. Increasing concentrations of S100b may stimulate the disassembly of intermediate filaments of astrocytes. The immunohistochemical analysis helps to reveal that astrocytes in the brain of rats that had HP lose the characteristic stellate shape and swelling. Immunoblotting result have shown the fragmentation of the main filament form of GFAP and appearance of low mass derivates. Application of 2-oxoglutarate (2.28 g/l of drinking water during 10 days after the onset of chronic hepatitis) stabilized the studied proteins and the state of astroglia.