Isolation and properties of lectins from cell walls of wheat seedlings infected by Fusarium graminearum and treated by salicylic acid

• Published in: Ukrains'kyi biokhimichnyi zhurnal (1999 ) Vol. 82; no. 5; p. 111
• Main Authors: Molodchenkova, O O, Adamovskaia, V G, Tsiselskaia, L Ĭ, Sagaĭdak, T V
• Format: Journal Article
• Language: Russian
• Published: Ukraine 01.09.2010
• Subjects: Cell Wall - metabolism; Cell Wall - microbiology; Chromatography, Affinity; Chromatography, Gel; Fusarium - drug effects; Fusarium - growth & development; Isoelectric Focusing; Lectins - biosynthesis; Lectins - isolation & purification; Lectins - pharmacology; Plant Diseases - microbiology; Plant Diseases - prevention & control; Plant Proteins - biosynthesis; Plant Proteins - isolation & purification; Plant Proteins - pharmacology; Plant Roots - metabolism; Plant Roots - microbiology; Protein Isoforms - biosynthesis; Protein Isoforms - isolation & purification; Protein Isoforms - pharmacology; Salicylic Acid - pharmacology; Seedlings - metabolism; Seedlings - microbiology; Triticum - metabolism; Triticum - microbiology

Lectins from cell walls of wheat seedlings in normal conditions and infected by Fusarium sp. and treated by salicylic acid were isolated by the methods of gel-filtration, affinity chromatography and isoelectric focusing, their biochemical properties were studied. It is shown that isolated preparations of lectins suppressed the development of colonies of fungus Fusarium graminearum. A conclusion was made on the possibility of employment of isolated lectins in the molecular-breeding programs on raising wheat resistance towards fusariosis.