Isolation and properties of lectins from cell walls of wheat seedlings infected by Fusarium graminearum and treated by salicylic acid

Lectins from cell walls of wheat seedlings in normal conditions and infected by Fusarium sp. and treated by salicylic acid were isolated by the methods of gel-filtration, affinity chromatography and isoelectric focusing, their biochemical properties were studied. It is shown that isolated preparatio...

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Published inUkrains'kyi biokhimichnyi zhurnal (1999 ) Vol. 82; no. 5; p. 111
Main Authors Molodchenkova, O O, Adamovskaia, V G, Tsiselskaia, L Ĭ, Sagaĭdak, T V
Format Journal Article
LanguageRussian
Published Ukraine 01.09.2010
Subjects
Cell Wall - metabolism
Cell Wall - microbiology
Chromatography, Affinity
Chromatography, Gel
Fusarium - drug effects
Fusarium - growth & development
Isoelectric Focusing
Lectins - biosynthesis
Lectins - isolation & purification
Lectins - pharmacology
Plant Diseases - microbiology
Plant Diseases - prevention & control
Plant Proteins - biosynthesis
Plant Proteins - isolation & purification
Plant Proteins - pharmacology
Plant Roots - metabolism
Plant Roots - microbiology
Protein Isoforms - biosynthesis
Protein Isoforms - isolation & purification
Protein Isoforms - pharmacology
Salicylic Acid - pharmacology
Seedlings - metabolism
Seedlings - microbiology
Triticum - metabolism
Triticum - microbiology
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Summary:Lectins from cell walls of wheat seedlings in normal conditions and infected by Fusarium sp. and treated by salicylic acid were isolated by the methods of gel-filtration, affinity chromatography and isoelectric focusing, their biochemical properties were studied. It is shown that isolated preparations of lectins suppressed the development of colonies of fungus Fusarium graminearum. A conclusion was made on the possibility of employment of isolated lectins in the molecular-breeding programs on raising wheat resistance towards fusariosis.