Comparison of NO production level and proliferation of MCF-7 breast tumor cells under conditions of microenvironment modification

• Published in: Ukrains'kyi biokhimichnyi zhurnal (1999 ) Vol. 82; no. 4; p. 60
• Main Authors: Perepelytsina, O M, Harmanchuk, L V, Shevchenko, Ie A, Sydorenko, M V, Ostapchenko, L I
• Format: Journal Article
• Language: Ukrainian
• Published: Ukraine 01.07.2010
• Subjects: Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Calcium - metabolism; Calcium Channel Blockers - pharmacology; Calcium Channels, L-Type - metabolism; Cell Culture Techniques; Cell Line, Tumor; Cell Proliferation; Culture Media; Female; Humans; Nitric Oxide - biosynthesis; Spheroids, Cellular - metabolism; Spheroids, Cellular - pathology

Connection between the level of NO generation and its cytotoxic influence was investigated on two models of tumor cells (breast adenocarcinoma MCF-7) culture: monolayer and spheroids. Conditions of microenvironment were modified by Ca2+ channels blockers: EGTA, nitrendipine and Cl-lanthan. It was discovered in the work that deviation in concentration of extracellular Ca2+ under the influence of EGTA leads to reducing of the level of extracellular NO to 50% (P < or = 0.05) of control and increasing of the number of live cells by 10-40% (P < or = 0.05). Nitrendipin has the properties selective Ca2+ channels blocker that reduced the level of NO by 35-70% (P < or = 0.05), depending on the substances concentration and time of incubation. At that time proliferation potential of cells increased by 10-35% (P < or = 0.05). NO production in MCF-7 cells under the influence of Cl-lanthan in concentration of 0.01 and 0.1 mM is reduced by 50-60%, as compared with the control. But it had no influence on the number of live cells. On 2-D and 3-D models of tumor growth it was demonstrated that extracellular NO production in monolayer of MCF-7 was 3.2 pmol for 10(6) cells and for spheroids it was 8.5 pmol.