Selection of naturally occurring autoantibodies to interleukin-18 from phage display library

• Published in: Human antibodies Vol. 19; no. 2-3; p. 71
• Main Authors: Vikhrova, M A, Shveygert, M V, Khrapov, E A, Filipenko, M L, Gileva, I P, Morozova, V V, Tikunova, N V
• Format: Journal Article
• Language: English
• Published: Netherlands 2010
• Subjects: Amino Acid Sequence; Autoantibodies - genetics; Autoantibodies - immunology; Blotting, Western; Enzyme-Linked Immunosorbent Assay; Humans; Immunoglobulin Heavy Chains - chemistry; Immunoglobulin Heavy Chains - genetics; Immunoglobulin Heavy Chains - immunology; Immunoglobulin Light Chains - chemistry; Immunoglobulin Light Chains - genetics; Immunoglobulin Light Chains - immunology; Interleukin-18 - genetics; Interleukin-18 - immunology; Molecular Sequence Data; Peptide Library; Polymorphism, Restriction Fragment Length; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Single-Chain Antibodies - genetics; Single-Chain Antibodies - immunology
• ISSN: 1875-869X

Four unique phage single-chain variable fragments (scFvs) to recombinant human interleukin 18 (IL-18) has been selected from a naïve combinatorial library of human scFvs. Binding of unique phage antibodies with IL-18 was tested by ELISA and Western-blotting. No cross reactivity with tumor necrosis factor α, interferons α and γ was shown for the selected antibodies. The gene segments encoding V(D)J regions of selected antibodies exhibited a high degree of homology to germline genes, therefore we suggest that the selected scFv's belong to repertoire of naïve autoantibodies.