Investigation of interferon-induced system of 2',5'-oligoadenylate in rat lymphoid cells exposed to ionizing radiation

• Published in: Ukrains'kyi biokhimichnyi zhurnal (1999 ) Vol. 76; no. 3; p. 126
• Main Authors: Mykhaĭlyk, I V, Prokopova, K V, Ostapchenko, L I, Kucherenko, M Ie
• Format: Journal Article
• Language: Ukrainian
• Published: Ukraine 01.05.2004
• Subjects: 2',5'-Oligoadenylate Synthetase - metabolism; Adenine Nucleotides - metabolism; Animals; Dose-Response Relationship, Radiation; Female; Gamma Rays; Interferon Inducers - pharmacology; Interferons - biosynthesis; Interferons - physiology; Lymphocytes - enzymology; Lymphocytes - metabolism; Lymphocytes - radiation effects; Male; Oligoribonucleotides - metabolism; Radiation Dosage; Rats; Rats, Wistar; Second Messenger Systems - physiology; Second Messenger Systems - radiation effects; Spleen - cytology; Spleen - enzymology; Spleen - metabolism; Spleen - radiation effects; Thymus Gland - cytology; Thymus Gland - enzymology; Thymus Gland - metabolism; Thymus Gland - radiation effects

The key components of interferon-dependent 2',5'-oligoadenylate system were studied in the rat spleen and thymus lymphoid cells under the action of ionizing radiation and interferon inducers treatment. It was established that the irradiation of animals by 0.5 Gy dose causes the increase of intracellular level of the 2',5'-oligoadenylates (A2'p5'A, (A2'p)2A) and the enhancement of the 2',5'-oligoadenylate-synthetase activity. The pre-incubation of isolated splenocytes and thymocytes with interferon inducers (cycloferone, mitogenic lectines and poly(I) x poly(C) caused the amplification of post-radiactive 2',5'-oligoadenylate accumulation and 2',5'-oligoadenylate-synthetase activity stimulation. The obtained results allow us to suggest the activation of 2',5'-oligoadenylate messenger system in rat immunocompetent cells under the action of ionizing radiation. It might be a result of the activation of protective mechanisms, connected with interferon induction. The effect of interferon inducers on the investigated cascade components may be considered as the manifestation of their radioprotective properties.