Investigation of interferon-induced system of 2',5'-oligoadenylate in rat lymphoid cells exposed to ionizing radiation

The key components of interferon-dependent 2',5'-oligoadenylate system were studied in the rat spleen and thymus lymphoid cells under the action of ionizing radiation and interferon inducers treatment. It was established that the irradiation of animals by 0.5 Gy dose causes the increase of...

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Bibliographic Details
Published inUkrains'kyi biokhimichnyi zhurnal (1999 ) Vol. 76; no. 3; p. 126
Main Authors Mykhaĭlyk, I V, Prokopova, K V, Ostapchenko, L I, Kucherenko, M Ie
Format Journal Article
LanguageUkrainian
Published Ukraine 01.05.2004
Subjects
2',5'-Oligoadenylate Synthetase - metabolism
Adenine Nucleotides - metabolism
Animals
Dose-Response Relationship, Radiation
Female
Gamma Rays
Interferon Inducers - pharmacology
Interferons - biosynthesis
Interferons - physiology
Lymphocytes - enzymology
Lymphocytes - metabolism
Lymphocytes - radiation effects
Male
Oligoribonucleotides - metabolism
Radiation Dosage
Rats
Rats, Wistar
Second Messenger Systems - physiology
Second Messenger Systems - radiation effects
Spleen - cytology
Spleen - enzymology
Spleen - metabolism
Spleen - radiation effects
Thymus Gland - cytology
Thymus Gland - enzymology
Thymus Gland - metabolism
Thymus Gland - radiation effects
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Summary:The key components of interferon-dependent 2',5'-oligoadenylate system were studied in the rat spleen and thymus lymphoid cells under the action of ionizing radiation and interferon inducers treatment. It was established that the irradiation of animals by 0.5 Gy dose causes the increase of intracellular level of the 2',5'-oligoadenylates (A2'p5'A, (A2'p)2A) and the enhancement of the 2',5'-oligoadenylate-synthetase activity. The pre-incubation of isolated splenocytes and thymocytes with interferon inducers (cycloferone, mitogenic lectines and poly(I) x poly(C) caused the amplification of post-radiactive 2',5'-oligoadenylate accumulation and 2',5'-oligoadenylate-synthetase activity stimulation. The obtained results allow us to suggest the activation of 2',5'-oligoadenylate messenger system in rat immunocompetent cells under the action of ionizing radiation. It might be a result of the activation of protective mechanisms, connected with interferon induction. The effect of interferon inducers on the investigated cascade components may be considered as the manifestation of their radioprotective properties.