Serologic diagnostics of Legionella infection

Technical approaches to construction of preparations for serologic diagnostics of Legionella infection were presented in the article; antigenic- and immunoglobulin-based diagnostic kits with known characteristics were developed. Immunogenic properties of protein and lypopolysaccharide antigens, whic...

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Bibliographic Details
Published inŽurnal mikrobiologii, ėpidemiologii i immunobiologii no. 2; p. 46
Main Authors Karbyshev, G L, Terent'ev, A N, Verkina, L M, Simonova, I R, Kochetkova, A P, Narkevich, A N, Lysova, L K, Larionova, L V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.2008
Subjects
Agglutination
Animals
Antibodies, Bacterial - immunology
Antibody Specificity
Antigens, Bacterial - analysis
Antigens, Bacterial - immunology
Bacterial Outer Membrane Proteins - immunology
Bacteriological Techniques - methods
Humans
Immune Sera
Immunoenzyme Techniques
Immunoglobulins
Legionella - immunology
Legionellosis - diagnosis
Legionellosis - microbiology
Legionellosis - urine
Lipopolysaccharides - analysis
Lipopolysaccharides - immunology
Microspheres
Polymers
Rabbits
Reagent Kits, Diagnostic - microbiology
Sensitivity and Specificity
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Summary:Technical approaches to construction of preparations for serologic diagnostics of Legionella infection were presented in the article; antigenic- and immunoglobulin-based diagnostic kits with known characteristics were developed. Immunogenic properties of protein and lypopolysaccharide antigens, which have diagnostic value, were studied; similarity of protein antigens from 7 serogroups of L. pneumophila was demonstrated. Soluble antigen with known composition was obtained and used for the development of antigen-based polymeric kit for diagnostics of Legionella infection. On the basis of hyperimmune sera, immunoglobulin-based polymeric diagnostic kit and array of coagglutinating diagnostic kits for the mentioned 7 serogroups were developed. Antigen-based polymeric diagnostic kit was recommended for licensure.
ISSN:0372-9311