Isolation of factor IX concentrate from cryosupernatant of donor's plasma

Soviet anion-exchangers were comparatively studied to specify an optimal carrier useful for isolation of factor ix of the blood coagulation system from donor's blood cryosupernatant. The main criteria of gel selection were the yield and purification degree of factor IX, as well as its specific...

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Bibliographic Details
Published inGematologiia i transfuziologiia Vol. 36; no. 6; p. 34
Main Authors Fetisova, L V, Viazova, E P, Khametova, R N, Shuvalova, A L, Azhigirova, M A, Lazarev, V A, Zeĭnalov, A M
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.06.1991
Subjects
Blood Donors
Blood Preservation - methods
Blood Transfusion
Chromatography, Ion Exchange - methods
Cryopreservation - methods
Factor IX - isolation & purification
Factor IX - therapeutic use
Hemophilia B - therapy
Humans
Immunoelectrophoresis, Two-Dimensional - methods
Male
Plasma - chemistry
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Summary:Soviet anion-exchangers were comparatively studied to specify an optimal carrier useful for isolation of factor ix of the blood coagulation system from donor's blood cryosupernatant. The main criteria of gel selection were the yield and purification degree of factor IX, as well as its specific activity and concentration in eluates. It has been shown that DEAE-agarose 6B-CL, possessing pronounced activity with respect to specific sorption of factor IX, meets these requirements to the greatest degree.
ISSN:0234-5730