Synthetic fragments of the NS1 protein of the tick-borne encephalitis virus exhibiting a protective effect

• Published in: Bioorganicheskaia khimiia Vol. 33; no. 2; p. 229
• Main Authors: Volkova, T D, Koroev, D O, Titova, M A, Oboznaia, M B, Filatova, M P, Vorovich, M F, Ozherelkov, S V, Timofeev, A V, Vol'pina, O M
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.03.2007
• Subjects: Amino Acid Sequence; Animals; Antibodies, Viral - blood; Encephalitis, Tick-Borne - prevention & control; Immunization; Mice; Mice, Inbred Strains; Molecular Sequence Data; Peptides - chemistry; Peptides - immunology; Peptides - therapeutic use; Viral Nonstructural Proteins - chemistry; Viral Nonstructural Proteins - immunology; Viral Nonstructural Proteins - therapeutic use
• ISSN: 0132-3423

Potentially immunoactive regions of the NS1 nonstructural protein of the tick-borne encephalitis virus that can stimulate the antibody formation in vivo and protect animals from this disease were chosen on the basis of theoretical calculations. Eleven 16- to 27-aa peptides containing the chosen regions were synthesized. The ability of the free peptides (without any high-molecular-mass carrier) to stimulate the production of antipeptide antibodies in mice of three lines and ensure the formation of protective immunity was studied. Most of these peptides were shown to exhibit the immunogenic activity in a free state. Five fragments that can protect mice from the infection by a lethal dose of tick-borne encephalitis virus were found.