13C NMR isotopomeric analysis and its application in the study of endocrine cell metabolism and function

Defining mechanisms and enzymatic paths critical to cellular function (e.g., secretion) of endocrine cells is a key research goal that can lead toward novel avenues of therapeutic intervention for a variety of disorders. 13C NMR spectroscopy and isotopomer analysis of cell extracts are excellent too...

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Bibliographic Details
Published inActa bio-medica de l'Ateneo Parmense Vol. 78 Suppl 1; p. 99
Main Authors Simpson, Nicholas E, Constantinidis, Ioannis
Format Journal Article
LanguageEnglish
Published Italy 2007
Subjects
Animals
Carbon Isotopes
Cell Line, Tumor - metabolism
Citric Acid Cycle
Culture Media
Endocrine System Diseases - metabolism
Energy Metabolism
Glutamic Acid - metabolism
Insulin - metabolism
Insulin Secretion
Insulin-Secreting Cells - metabolism
Insulin-Secreting Cells - pathology
Insulinoma - pathology
Models, Biological
Nuclear Magnetic Resonance, Biomolecular - methods
Pancreatic Neoplasms - pathology
Pyruvates - metabolism
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Summary:Defining mechanisms and enzymatic paths critical to cellular function (e.g., secretion) of endocrine cells is a key research goal that can lead toward novel avenues of therapeutic intervention for a variety of disorders. 13C NMR spectroscopy and isotopomer analysis of cell extracts are excellent tools to quantitatively assess metabolism through intermediate labeling and estimate carbon entry to the TCA cycle. Discussed are: cell lines and in vitro culturing; extraction of intracellular material; NMR spectroscopy of the extract; isotopomeric analysis and modeling to obtain relative metabolic fluxes to the TCA cycle. This paper describes issues related to the application of NMR spectroscopic techniques on cell line extracts. Included are results of two studies that illustrate considerations that must be taken when performing analogous studies on neuroendocrine tissue: one involving the effect of media composition on cell behavior and isotopomer labeling; the second looking at effects of applying different metabolic models to 13C data and inferences that may be drawn. NMR isotopomeric analysis is a powerful technique that may be applied to better understand endocrine cell function.
ISSN:0392-4203