Detection of Pneumocystis carinii DNA in air and washes from medical equipment in hospitals

Results of study of rooms' air and washes from medical equipment by PCR assay to detect Pneumocystis carinii DNA are presented. PCR assay sensivity was 200 copies/ml. Method of taking of air samples by MC-2 sample-taking device was modified for P. carinii detection. Sensivity of the method was...

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Bibliographic Details
Published inŽurnal mikrobiologii, ėpidemiologii i immunobiologii no. 7; p. 100
Main Authors Kunakbaeva, A F, Karazhas, N V, Zigangirova, N A, Rybalkina, T N, Galstian, G M, Osmanov, E A, Petrosov, V V, Bosh'ian, R E, Savitskaia, N V, Feklisova, L V, Iuditskiĭ, M V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.11.2006
Subjects
Air Microbiology
Attention
Colony Count, Microbial
DNA, Fungal - analysis
Environmental Exposure - analysis
Equipment and Supplies, Hospital - microbiology
Pneumocystis carinii - genetics
Pneumocystis carinii - isolation & purification
Pneumocystis Infections - prevention & control
Polymerase Chain Reaction
Sensitivity and Specificity
Specimen Handling - instrumentation
Specimen Handling - methods
Vacuum
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Summary:Results of study of rooms' air and washes from medical equipment by PCR assay to detect Pneumocystis carinii DNA are presented. PCR assay sensivity was 200 copies/ml. Method of taking of air samples by MC-2 sample-taking device was modified for P. carinii detection. Sensivity of the method was 10 copies/m3. 27 air samples and 105 washes from medical equipment were studied and P. carinii DNA was not detected. It has been shown during the study that DNA of pneumocysts remains intact at room temperature during 12 days including 2-hour ultraviolet (UV) radiation treatment. After processing of studied surfaces with 0.1% solution of chloramine with subsequent UV radiation treatment during 30 minutes results of PCR assay were negative.
ISSN:0372-9311