The determination of antigen-specific immune complexes by an immunoenzyme method

The methodological approach permitting the detection of immune complexes containing specific antibodies to a definite antigen in the enzyme-linked immunosorbent assay (ELISA) is described. The basic conditions of the assay were optimized. Immune complexes were precipitated from blood serum with 3.5%...

Full description

Saved in:
Bibliographic Details
Published inŽurnal mikrobiologii, ėpidemiologii i immunobiologii no. 1; p. 67
Main Authors Bobrovnik, S A, Liashchenko, K P
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.1991
Subjects
Animals
Antibodies, Bacterial - blood
Antibodies, Bacterial - isolation & purification
Antigen-Antibody Complex - blood
BCG Vaccine - immunology
Cattle
Enzyme-Linked Immunosorbent Assay - methods
Epitopes - blood
Female
Immune Sera - isolation & purification
Immunization
Mycobacterium tuberculosis - immunology
Tuberculin - immunology
Online AccessGet more information

Cover

More Information
Summary:The methodological approach permitting the detection of immune complexes containing specific antibodies to a definite antigen in the enzyme-linked immunosorbent assay (ELISA) is described. The basic conditions of the assay were optimized. Immune complexes were precipitated from blood serum with 3.5% polyethylene glycol 6000 for 4 hours. The precipitate thus obtained was dissolved and incubated in polystyrene plates with immobilized antigen at 37 degrees C for a long time (at least 6 hours) in a humid chamber. The amount of bound antibodies, determined by ELISA techniques, was conjectured from the level of antigen-specific immune complexes. The proposed approach can be used in the immunodiagnosis of infectious diseases.
ISSN:0372-9311