Inhibitory effect of bladder cancer related protein gene on HeLa cell proliferation

• Published in: Ai zheng = Aizheng = Chinese journal of cancer Vol. 25; no. 7; p. 811
• Main Authors: Zuo, Ze-Hua, Zhao, Min, Liu, Juan, Wei, Yun, Wu, Xin-Xing
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2006
• Subjects: Animals; Apoptosis; Cell Proliferation; Cervix Uteri - metabolism; Female; Gene Expression Regulation, Neoplastic; Genes, Tumor Suppressor; Genetic Vectors; HeLa Cells; Humans; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; Neoplasm Proteins - physiology; Neoplasm Transplantation; Transfection; Uterine Cervical Neoplasms - metabolism; Uterine Cervical Neoplasms - pathology

Bladder cancer related protein (BLCAP) gene was found downregulated most in primary cervical cancer tissues using oncogene/tumor suppressor gene microarray screening in our previous study, therefore this study was to explore the possible correlation between BLCAP gene and cervical cancer. BLCAP expression was investigated in 54 cervical cancer and 25 normal tissues by reverse transcription polymerase chain reaction (RT-PCR). Full length of BLCAP cDNA was cloned into pLXSN expression vector and stably transfected into cervical cancer cell line HeLa cells. Cell proliferation, colony formation ability and apoptosis were determined by cell number counting, colony formation and DNA ladder assay. Nude mice were used to study the anti-tumor effect of BLCAP gene in vivo. BLCAP gene expression was significantly down-regulated or even not observed in human cervical cancer tissues compared to the normal ones. The cell doubling time of HeLa cells transfected with BLCAP was significantly elevated to 69.4 hrs (P<0.05), com