Monoclonal antibodies to Rho(D)-antigen of the Rhesus system. Obtaining the EBV-transformed B-lymphoblastoid human cell lines secreting IgG-anti-D antibodies

• Published in: Gematologiia i transfuziologiia Vol. 36; no. 8; p. 11
• Main Authors: Deriugina, E I, Olovnikova, N I, Lemeneva, L N, Belkina, E V, Udalov, G A, Chertkov, I L
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.08.1991
• Subjects: Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - immunology; B-Lymphocytes - cytology; B-Lymphocytes - immunology; Biotechnology - methods; Cell Line, Transformed; Cell Transformation, Viral - immunology; Cells, Cultured; Herpesvirus 4, Human - physiology; Humans; Immunoglobulin G - biosynthesis; Immunoglobulin G - immunology; In Vitro Techniques; Isoantibodies - biosynthesis; Isoantibodies - immunology; Rh-Hr Blood-Group System - immunology

Human B-lymphoblastoid cell lines producing specific anti-D antibodies against Rh0(D) antigen have been established by EBV-transformation. The strong influence of the donor's immune status (serum titer of anti-D antibodies, intravenous booster injection prior to bleeding) is shown. The cell lin...