Monoclonal antibodies to Rho(D)-antigen of the Rhesus system. Obtaining the EBV-transformed B-lymphoblastoid human cell lines secreting IgG-anti-D antibodies

• Published in: Gematologiia i transfuziologiia Vol. 36; no. 8; p. 11
• Main Authors: Deriugina, E I, Olovnikova, N I, Lemeneva, L N, Belkina, E V, Udalov, G A, Chertkov, I L
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.08.1991
• Subjects: Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - immunology; B-Lymphocytes - cytology; B-Lymphocytes - immunology; Biotechnology - methods; Cell Line, Transformed; Cell Transformation, Viral - immunology; Cells, Cultured; Herpesvirus 4, Human - physiology; Humans; Immunoglobulin G - biosynthesis; Immunoglobulin G - immunology; In Vitro Techniques; Isoantibodies - biosynthesis; Isoantibodies - immunology; Rh-Hr Blood-Group System - immunology
• ISSN: 0234-5730

Human B-lymphoblastoid cell lines producing specific anti-D antibodies against Rh0(D) antigen have been established by EBV-transformation. The strong influence of the donor's immune status (serum titer of anti-D antibodies, intravenous booster injection prior to bleeding) is shown. The cell lines cloned by limiting dilution continuously produce anti-D antibodies in culture for 7 months or longer which is sufficient to support large-scale production of anti-D antibodies based on the cultivation of cryopreserved cells. Anti-D IgGl monoclonal antibodies obtained react with Rh-positive and Du cells but not with Rh-negative cells in serological tests. These human monoclonal antibodies could be useful in the prevention of Rh hemolytic disease of newborns.