Cultivation of Mycobacterium tuberculosis in cell culture

The method of the cultivation of M .tuberculosis in cell subcultures was tested. Five consecutive passages of 2 M. tuberculosis strains were carried out with similar inoculation and cultivation conditions. Mycobacterial cells preserved their morphological characteristics in the course of all passage...

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Bibliographic Details
Published inŽurnal mikrobiologii, ėpidemiologii i immunobiologii no. 4; p. 63
Main Authors Alekseev, A Iu, Durymanov, A G, Rassadkin, Iu N, Repin, V E, Shestopalov, A M
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.07.2004
Subjects
Animals
Cercopithecus aethiops
Culture Media
Mycobacterium tuberculosis - growth & development
Time Factors
Vero Cells
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Summary:The method of the cultivation of M .tuberculosis in cell subcultures was tested. Five consecutive passages of 2 M. tuberculosis strains were carried out with similar inoculation and cultivation conditions. Mycobacterial cells preserved their morphological characteristics in the course of all passages. The method of M. tuberculosis cultivation in subcultured cells made it possible to study of the physiology of M. tuberculosis under conditions, most approximated to the natural tuberculosis infection in humans.
ISSN:0372-9311