Effect of N-stearoylethanolamine and ionizing radiation on lipid components of the liver and heart microsomes in rats

• Published in: Ukrains'kyi biokhimichnyi zhurnal (1999 ) Vol. 75; no. 4; p. 81
• Main Authors: Artamonov, M V, Zhukov, O D, Horid'ko, T M, Klimashevs'kyĭ, V M, Martseniuk, O P, Hula, N M
• Format: Journal Article
• Language: Ukrainian
• Published: Ukraine 01.07.2003
• Subjects: Administration, Oral; Animals; Ethanolamines - administration & dosage; Ethanolamines - pharmacology; Heart - drug effects; Heart - radiation effects; Microsomes - drug effects; Microsomes - metabolism; Microsomes - radiation effects; Microsomes, Liver - drug effects; Microsomes, Liver - metabolism; Microsomes, Liver - radiation effects; Organ Specificity; Phospholipids - metabolism; Radiation-Protective Agents - administration & dosage; Radiation-Protective Agents - pharmacology; Rats; Rats, Wistar; Stearic Acids - administration & dosage; Stearic Acids - pharmacology; Whole-Body Irradiation; X-Rays

The influence of irradiation and NSE on the microsomal lipid composition of the rat liver and heart was studied. It was shown, that radiation treatment in a dose of 2 Gy had not a significant affect on the heart phospholipid composition, whereas in the liver the amounts of the phosphatidylethnolamine and phosphatidylinositol were increased and the amounts of the phosphatidylcholine and sphyngomieline were decreased. NSE did not impact on these characteristics. The alterations of the plasmalogen/diacyl forms ratio of the PC and PE also took place only in the liver microsome. The analysis of the fatty acids esterified to phospholipids showed similar situation: the fatty acids of the heart microsome less were changed than those of the liver microsome undergo irradiation. The amount of arachidonic acid rise after the adding of NSE to rats. Investigation of the important component of biological membranes--cholesterol and its esters also showed that the liver tissue is more vulnerable to irradiation than the heart. NSE led to insignificant increase of the not esterified cholesterol in the liver. As a result of the present work we may conclude that the liver microsome is more vulnerable than the heart under X-ray radiation and NSE has protective effect in these conditions.