Expression and significance of nitric oxide synthase mRNAs and proteins in giant cell tumors of bone

• Published in: Ai zheng = Aizheng = Chinese journal of cancer Vol. 22; no. 11; p. 1180
• Main Authors: Li, Hai-Gang, Pan, Chao-Bin, Zeng, Hong, Zeng, Yun-Jie, Shen, Xi-Ming, Dong, Shu-Kun
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2003
• Subjects: Adolescent; Adult; Bone Neoplasms - enzymology; Bone Neoplasms - pathology; Female; Giant Cell Tumors - enzymology; Giant Cell Tumors - pathology; Humans; Male; Middle Aged; Neoplasm Recurrence, Local; Neoplasm Staging; Nitric Oxide Synthase - genetics; RNA, Messenger - analysis

Recent studies have indicated that nitric oxide (NO) plays an important role in carcinogenesis and tumor progression. Activity of nitric oxide synthase (NOS) has been detected in normal bone cell lines. There was no report about relation of expression of NOS in giant cell tumors(GCT) of bone with its pathological grading and tumor recurrence. This study was designed to investigate the relationship amoung expression of NOS mRNAs, NOS proteins, pathological grading and tumor recurrence. In situ hybridization (ISH) with cDNA probe was used to determine 14 frozen GCT specimens for constitutive NOS(cNOS) mRNA and inducible NOS(iNOS) mRNA. Immunohistochemical (IHC) staining with multiclonal antibodies was used to determine 42 paraffin-embedded GCT specimens for protein expression of NOS1, NOS2, and NOS3. (1)In 14 frozen GCT specimens, the positive expression rates of cNOS and iNOS mRNAs of multinuclear giant cells (MGC) were 78.6% and 57.1%; the positive expression rates of cNOS and iNOS mRNAs in mononuclear cells