A composite polyaniline-containing silica sorbent for DNA isolation

A composite sorbent based on porous glass beads modified with thin polyaniline coating was prepared by precipitating aniline polymerization in the presence of carrier particles. It was shown that the modification ensures the uniform coating of the inner surface of the carrier pores with the polymer...

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Published inBioorganicheskaia khimiia Vol. 29; no. 3; p. 310
Main Authors Kapustin, D V, Iagudaeva, E Iu, Zavada, L L, Zhigis, L S, Zubov, V P, Iaroshevskaia, E M, Plobner, L, Leiser, R M, Brem, G
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.2003
Subjects
Aniline Compounds - chemistry
Apoptosis - genetics
Apoptosis - radiation effects
Biochemistry - methods
Carcinoma - genetics
Carcinoma - pathology
Chromatography - methods
Colonic Neoplasms - genetics
Colonic Neoplasms - pathology
DNA - chemistry
DNA - isolation & purification
DNA, Bacterial - isolation & purification
DNA, Neoplasm - isolation & purification
Electrophoresis, Polyacrylamide Gel
Escherichia coli - genetics
Humans
Silicon Dioxide - chemistry
Ultraviolet Rays
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Summary:A composite sorbent based on porous glass beads modified with thin polyaniline coating was prepared by precipitating aniline polymerization in the presence of carrier particles. It was shown that the modification ensures the uniform coating of the inner surface of the carrier pores with the polymer layer approximately 70 A thick. It was shown that the resulting material retains the initial porosity of the carrier and is selective in the separation of nucleic acids and proteins. The polyaniline-coated sorbents were shown to be efficient for both the preparative DNA isolation from bacterial lysates and for analytical purposes, in particular, for studying DNA fragmentation during apoptosis proceeding under UV irradiation of cell lysates of colon carcinoma. The morphological and chromatographic characteristics of the new sorbent were demonstrated to be similar to those of the polyfluorobutadiene sorbent.
ISSN:0132-3423